Abstract
1. 1. A partially purified hemolytic fraction was obtained from a crude preparation of M. pyogenes var. aureus toxin by dialysis and precipitation with 20 per cent acetone. 2. 2. A respiratory acceleration previously reported in the crude toxin was removed by dialysis. 3. 3. Prior to hemolysis there is no loss of lipid from the membranes of chicken erythrocytes exposed to this toxin. 4. 4. The permeability of these cells to glycerol is unaffected. 5. 5. The present data are not incompatible with the hypothesis that these cells hemolyze in the presence of this toxin by colloidal osmotic hemolysis.
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