Abstract
The periodic acid-Schiff (PAS) reaction has been studied in sections of mouse liver using a self-assembled microspectrophotometer. Increased colour intensity was obtained up to 4 hr of oxidation with periodic acid and 2 hr treatment with Schiff's reagent. The oxidation curve showed an initial, steep increase in colouration with a levelling off afterwards, that could not be attributed to loss of aldehyde groups. The results obtained from carrying out the PAS reaction on sections pretreated with β-amylase suggest that the reaction takes place in two phases in which the outer glucosyl groups of the glycogen molecule are oxidized more rapidly than the inner ones.
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