Abstract
Simple calculations show that logarithmic amplifiers for spectroscopic detectors are not normally necessary. The area of a peak measured by a detector without logarithmic amplifier differs by less than 0.3% from that measured by a logarithmic amplifier for the concentrations used in high speed liquid chromatography. Double beam instruments have been found to give no improvement in noise level and drift over single beam instruments. Noise and drift of both is mainly caused by the photodiodes having (1–5)·10−4 absorbance. The difficulties involved by using a reference cell flow in gradient elution techniques where the eluent also absorbs are described briefly. It is concluded that properly designed commercial single beam instruments without logarithmic amplifiers suit the demands for high speed liquid chromatography completely.
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