Abstract
Purified Saliva-parotin-A, almost homogeneous electrophoretically and ultracentrifugally, consisted of 18 kinds of amino acids. Its biological activities were extremely powerful and the minimum effective dose for rabbit serum calcium decreasing activity was 5-10μg, for circulating leucocytes activity 0.25-0.5μg and for calcification of rabbit incisor dentine 2μg.The biological activities were extremely stable in acid and neutral solutions but comparatively unstable in alkaline solution.It was partly digested by proteolytic enzymes without loss of its biological activities. It was inactivated by formaldehyde but not by nitrous acid, iodine, thioglycollic acid or cysteine.It was not denatured by urea nor by guanidine.Amino, tyrosine-phenyl and sulfhydryl groups in Saliva-parotin-A molecule were not responsible for revealing its biological activities.
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