Abstract

Continuous synthesis of oleic acid glycerides by seven lipases available commercially were compared at 40°C using the microporous hydrophobic membrane bioreactor we developed recently. The lipases produced by Rhizopus japonicus, Candida cylindracea, and Phycomyces nitens were quickly inactivated in 97% glycerol solution and yielded very low glyceride conversions. The other four lipases yielded glyceride synthesis activities in the order: Rhizopus delemer’s<Pseudomonas fluorescens’s< Mucor miehei’s<Chromobacterium viscosum’s. For these four lipases, there was a hyperbola-like relationship between the conversion and the amount of lipase adsorbed at the membrane during the reaction. The adsorbed lipase desorbed relatively easily when glycerol-water-lipase solution was replaced by fresh 97% glycerol solution having no lipases. Mucor miehei’s lipase was adsorbed more strongly than Chromobacterium viscosum’s. The product produced by Mucor miehei’s lipase was composed mostly of mono- and di-glycerides while the one produced by Pseudomonas fluorescens’s was composed of mono-, di-, and tri-glycerides with an approximate molar ratio of 3:4:1. The contents of 2-monoglyceride and 1,2-diglyceride isomers in the product having about 80% conversion produced by Mucor miehei’s lipase were quite small. The approximate half-life of Mucor miehei’s lipase was estimated as 54 days from a continuous reaction experiment carried out for 27 days.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.