Some biochemical aspects of neurotubule and neurofilament proteins.

Abstract

In the few years since the isolation of the subunit protein of microtubules (1, 2, 23, 26) work on its chemistry, localization and metabolism has proceeded at a feverish pace. In their initial studies Taylor and his co-workers noted exceptionally high levels of colchicine-binding activity in brain homogenates and in axoplasm. Weisenberg, Borisy and Taylor (31) developed a simple procedure for the purification of the microtubule subunits (tubulin) from brain enabling investigators to use large quantities of highly purified protein for biochemical studies. Studies on metabolism and distribution of tubulin in brain were aided by the observation that vinblastine quantitatively and selectively precipitated tubulin from 100,000 g supernatants of brain homogenates (17, 18, 32).KeywordsSynaptic VesicleSodium BorohydrideCyanogen BromideTubulin SubunitColchicine BindingThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.