Abstract
Elloramycin was chosen as a representative anthraquinone antibiotic to determine the separation efficiency of preparative reversed-phase liquid chromatography with regard to dependency on sample solubility, sample solvent strength, sample concentration and particle size of the stationary phase. In the case of elloramycin, which shows moderate lipophilic properties, it was demonstrated that an optimal strength of the sample solvent avoided front elution and peak fronting, that a diluted sample led to a better separation, and that a small particle size like 10 μm resulted in a strong increase in the purity of the separated compound in contrast to low-cost 15–25-μm particles.
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