Somatostatin expression in the cerebellar cortex during postnatal development

Abstract

The distribution of somatostatin-immunoreactive (SOM-IR) elements in the cerebellar cortex of the rat has been studied at different stages of postnatal development (from birth to day 30) and in adult animals using immunohistochemistry. The results showed that in vermis of new born animals there are three main groups of SOM-IR structures within the cortex which subsequently spread along the Purkinje cell layer. In addition, both in the vermis and in the lateral lobes, numerous more evenly distributed SOM-positive cells and fibers could be seen. SOM-IR Golgi cells, Purkinje cells and climbing fibers could then be recognized during the subsequent developmental stages. In the vermal zone, SOM-IR Purkinje cells formed patches, which seemed to be part of a sagittal columnar or band-like organization. This was most obvious between days 5 and 21 of postnatal development. Subsequently there was a reduction in the number of immunoreactive Purkinje cells but a patchy disposition remained. In addition high numbers of SOM-IR Purkinje and Golgi cells and also climbing fibers were identified in the flocculus and paraflocculus at all stages of development studied, and they were also seen in the adult rats in these regions. In the lateral lobes expression of SOM-like immunoreactivity (LI) decreased and almost completely disappeared in adult animals. The present results demonstrate that a SOM or a SOM-LI peptide can be transiently detected in many Purkinje and Golgi cells in the cerebellar cortex, suggesting a role in events related to developmental processes. However, in some regions and structures SOM-LI can be seen also in adult animals.