Somatostatin 14- and somatostatin 25-like peptides in pancreatic endocrine cells of Sparus aurata (Teleost): A light and electron microscopic immunocytochemical study

Abstract

An immunocytochemical investigation demonstrates the presence of somatostatin (SST) 14- and salmon somatostatin (sSST) 25-like peptides in two populations of somatostatin (D) cells in the islets of gilthead sea bream ( Sparus aurata). Both cell types were identified by their differing immunoreactivities to the somatostatin antisera used. D1 cells in the islet periphery between glucagon cells showed sSST 25-like immunoreactivity and contained large moderate to low electron-dense granules. D2 cells, present only in the central region of the islets between insulin cells, were immunoreactive to the SST 14 antisera and had smaller electron-dense granules. In S. aurata, as in other teleosts, preprosomatostatin I and II are probably synthesized and processed to SST 14- and sSST 25-like peptides, respectively, in different D cell types.