Abstract
Somatomedin-C (Sm-C) was characterized in serum-free conditionned medium of porcine Sertoli cells cultured for 1 day in the presence of Transferrin, Vitamin E, Insulin and 3H-Leucine. Sm-C was purified by Sep-pak extraction followed by monoclonal anti-Sm-C antibody affinity chromatography and reverse phase CIS HPLC. Immunoreactive (IR) 3H-Leu-Sm-C co-eluted with pure 125-I-Sm-C on HPLC, gives a good parallelism to pure Sm-C in a specific radioimmunoassay. 48 hours IR-Sm-C secretion by Sertoli cells in serum-free conditionned medium (without insulin and 3H-Leu) is as follow : Conclusions. Sertoli cells secrete Somatomedin-C, in quantity compatible with an autocrine-paracrine role in the testis. Fibroblast Growth Factor (FGF) but not GH stimulates this secretion, as reported with fibroblasts.
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