Abstract
Somatic hybrids were produced between three processing tomato ( Lycopersicon esculentum) lines and Lycopersicon hirsutum. A simple fusion and culture procedure permitted the regeneration of 18 hybrids after fusion between leaf protoplasts of the wild species and etiolated hypocotyl protoplasts of three processing tomato lines. The hybrids displayed intermediate morphological characteristics for leaf shape and size, trichome type and density, fruit shape and color, and flower structure. A majority of the hybrids contained 2 n = 48 chromosomes and a nuclear DNA content approximating that of the sum of both parents'. All hybrids had inherited the cpDNA from the wild species but contained mtDNA from both parents. Some of the hybrids were fertile and produced abundant fruit and seed upon selfing.
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