Abstract
One of the efforts to increase domestic production is improving superior soybean through plant breeding techniques. Improving superior soybean cannot be separated from the regeneration process. Regeneration via somatic embryo induction has the advantage of being bipolar. Types of explants, genotypes, media composition, and growth regulators play an important role in plant in vitro regeneration. This study aims to obtain a regeneration protocol for two varieties of soybeans, namely, Derap I and Devon I, through induction of somatic embryos. This research was conducted at Tissue Culture Laboratory of the Faculty of Agriculture, Andalas University, Padang, from July to November 2020. This study used a completely randomized design (CRD) that was carried out separately in one experiment with 4 levels of 2,4-D, namely, 10 ppm, 20 ppm, 30 ppm, and 40 ppm, which were repeated 5 times. The results showed that the concentration of 2,4-D 20 ppm was the best concentration for percentage of explants that produced somatic embryos in Derap I (35%), and Devon I (95%).
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