Abstract
Immature embryos of 12 genotypes of Zea mays L. were cultured on agar nutrient media containing various concentrations of 2,4-dichlorophenoxyacetic acid and sucrose. An opaque, white, compact and organized callus tissue was formed by the proliferation of the scutellar cells present in the vicinity of the scutellar node near the coleorhiza. Well-organized somatic embryos were formed on the surface of the proliferating scutellar callus within about three weeks. The embryos showed the typical organization of grass embryos with a scutellum, coleoptile and coleorhiza, and germinated to form normal green plants which were grown to maturity. An embryogenic cell suspension culture obtained from the scutellar callus is now being used for the isolation and culture of protoplasts.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
