Abstract
The development of embryoids from leaf explants of Apium graveolens var. dulce cultured on Murashige and Skoog medium with 0.5 mg/l 2,4-D and 0.6 mg/l kinetin was followed. Four stages were recognized: (1) cell proliferation, (2) pro-embryoid formation, (3) torpedo embryoid formation and (4) plantlet formation. All embryoids were derived from the cortical tissues of leaf explants; no embryoids formed in the epidermal tissues.
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