Abstract
ABSTRACT The formation of somatic embryos in higher plants in vitro is an exogenously regulated process which can be divided into (a) the induction phase and (b) the release of induced cells for further development. There are, however, many examples in the literature where induction of somatic embryogenesis is restricted to certain genotypes or cultivars. The solution for overcoming the regeneration problem in these cases requires laborious and time-consuming try-and-error approaches, including the testing of numerous media and growth conditions, with unpredictable success. Since in most plant species, including cereals and seed legumes, the induction of somatic embryogenesis is a strictly auxin-controlled process, we studied the signal transduction pathway, focusing on the primary interaction site, namely auxin recognition. This requires at the beginning the characterization of specific auxin receptors in explants exhibiting embryogenic potential. In immature zygotic pea embryos and shoot tips of etiolat...
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