Abstract
Embryogenesis and organogenesis were achieved from callus tissue of anthers of Actinidia chinensis Planch. var. chinensis and Actinidia deliciosa (A. Chev.) Liang and Ferguson var. deliciosa. Anthers containing uninucleate or mitotic microspores were chilled for 7 days at 4°C and cultured on a basic nutrient medium supplemented with the cytokinin 2-isopentenyladenine and the auxin indoleacetic acid for highest yields of embryoids. The embryogenic potential of anthers was dependent on genotype, and male source plants yielded the largest numbers of embryoids. Sectioning of anthers demonstrated the somatic origin of the embryoids, and the root tip squashes from plant-lets grown from the embryoids showed a chromosome number of 58 (2 n) for A. chinensis and 170 (2 n) for A. deliciosa.
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