Abstract
Abstract Somatic embryogenesis was observed on cacao (Theobroma cacao L.) expiants obtained from white to white‐pink zygotic embryos of variety ‘Amelonado’ cultured on media supplemented with napthalene acetic acid (NAA), 1–32 μM, after six weeks of culturing. A high incidence of development‐arrested somatic embryogenesis (50%) occurred with expiants cultured in Murashige and Skoog medium containing 32 uM NAA, 0.10% casein hydrolysate and 3.0% sucrose. Embryogenesis rates were different among zygotic embryo expiants from different ‘Mexico’ strains or different pods of the ‘Amelonado’ variety. The regeneration of a plantlet via direct somatic embryogenesis from the hypocotyl surface of immature cacao zygotic embryos was observed.
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