Abstract
The present study describes plant regeneration via somatic embryogenesis from cotyledonary node callus. Callus cultures were derived from cotyledon node segment excised from Trigonella foenum-graecum L. axenic seedlings grown in agar solidified Murashing and Skoog's(MS) medium supplemented with Benzyl adenine BA at 2.0 mg l-1 and Naphthalene acetic acid NAA at 1.0 mg l-1. These cultures were stimulated to form somatic embryoids at 32% frequency of in agar-solidified MS medium containing NAA at 0.5mg l-1 in the presence of 15% v/v coconut water. Light microscopy examination of sections of heart embryos stage proved the presence of vascular system. Transfer of other developmental stages of embryos to the same induction medium led to the development of secondary somatic embryos and embryoids into plantlets in agar-solidified MS medium lacking hormones. They were readily rooted in MSO and successfully transferred to soil.
Highlights
Plant tissue culture systems have been developed for Fenugreek Trigonella foenumgraecum L., which utilize either shoot morphogenesis ]1[ or somatic embryogenesis ]2[as the route regeneration
Somatic embryogenesis phenomenon based on two steps, the first include the induction of embryogenic competence, in the presence of high concentration of auxin
There are exceptional cases, like carrot, where auxin did not required for somatic embryogenesis and its presence can inhibit the somatic embryogenesis, and embryo development may be achieved in absence or in presence of lower auxin and cytokinin concentration [9]
Summary
Somatic embryogenesis and plant regeneration from cotyledonary node's calli of Trigonella foenum-graecum L. جكٌين الأجنة انجسمية ًإخلاف اننباجات من كانس انؼقذ انفهقية ننبات انحهبة Trigonella foenum-graecum L. College of Engineering / University of Diyala * College of Education / University of Mosul ** College of Agriculture and Forestry /University of Mosul. جامعة ديالى/ كلية الهندسة جامعة المىصل/ *كلية التربية جامعة المىصل/ ** كلية الزراعة والغابات
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