Somatic antigens of Pseudomonas aeruginosa. The structure of O-specific polysaccharide chains of the lipopolysaccharides from P. aeruginosa II (Sandvik) and V (IM-1, Verder-Evans)

Abstract

The lipopolysaccharides from two serologically related strains of Pseudomonas aeruginosa II (Sandvik classification) and V (IM-1, Verder-Evans classification) were isolated by the Westphal method and cleaved with 1% acetic acid. The O-specific polysaccharide from Sandvik II lipopolysaccharide involved L-rhamnose, N-acetyl-D-quinovosamine, and N-acetyl-D-galactosaminuronic acid in the ratio 2:1:1, as well as O-acetyl groups. The Verder-Evans V O-specific polysaccharide was found to contain just the same sugars and, in addition, D-glucose, the monosaccharide ratio being 2:1:1:1. On the basis of methylation analysis, Smith degradation, solvolysis with anhydrous hydrogen fluoride and 1H and 13C nuclear magnetic resonance data, it was concluded that the polysaccharides have identical main chains, and there were established the following structures of their repeating units: -4)-alpha-D-GalNAcA-(1-3)-beta-D-QuiNAc-(1-2)-alpha-L-Rha-(1 -3)-alpha-L-Rha-(1- increases 3 60% OAc Sandvik II -4)-alpha-D-GalNAcA-(1-3)-beta-D-QuiNAc-(1-2)-alpha-L-Rha-(1 -3)-alpha-L-Rha-(1- increases 3 alpha-D-Glc Verder-Evans V The data obtained allow us to substantiate the combining of the strains studied into one O-serogroup as two individual O-serotypes.