Abstract
The use of oxygen-l7-enriched water as a relaxation agent provides a convenient means to suppress the water peak in proton NMR spectra. Results of using oxygen-I7-enriched water to study the proton NMR of an aqueous solution of angiotensin 11 acetate are presented. The Carr-Purcell-Meiboom-Gill (CPMG) sequence is used to attenuate the water peak, and all solute peaks are observed without interference. For 2D experiments, the CPMG spin-echo train is inserted after the first 90° pulse, and excellent 2D COSY, NOESY, and ROESY spectra have been obtained. A comparison of various water-suppression methods and the advantages of using oxygen-l7-enriched water as a relaxation agent are discussed.
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