Abstract
The concept and theory of solvent modulation are extended to serially coupled columns in liquid chromatography. Because the mobile phases are maintained in separate zones and the stationary phases in separate columns, solute retention is a simple, time-weighted average of the individual environments to which the solute is exposed. Thus, optimization of complex multidimensional chromatographic separations is more accurate and predictable. This approach is demonstrated by application to the separation of isomeric polynuclear aromatic hydrocarbons using octadecylsilica and β-cyclodextrin silica stationary phases with aqueous methanol and acetonitrile mobile phases.
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