Abstract
The new prototype device is applied to the Solvent Front Position Extraction (SFPE) sample preparation procedure. The mobile phase is deposited onto the chromatographic plate adsorbent layer by the pipette, which is moved, according to programmed movement path, by a 3D printer mechanism. The application of the prototype device to SFPE procedure leads to the increased repeatability of the results and significant reduction of the analysis time in comparison to the classical procedure of chromatogram development. Additionally, the new equipment allows use procedures that are not possible to run using the classic chromatogram development. In this paper, the results of manual and semi-automatic sample preparation with SFPE are compared and the possible application of this prototype device is discussed.
Highlights
Sample preparation is an integral part of any analytical method, influences all subsequent steps of the analysis, and has a relevant impact on the precision and accuracy of the results [1]
We presented a solvent front position extraction (SFPE) technique, which is based on planar solid phase extraction clean-up concept for pesticide residue analysis developed by Oellig and Schwack [29,30]
The necessity of cutting the plates into fragments corresponding to the sample track followed by chromatogram development from two opposite sides along the short edge of the plate towards its middle considerably prolongs the time of the procedure and makes it quite tedious
Summary
Sample preparation is an integral part of any analytical method, influences all subsequent steps of the analysis, and has a relevant impact on the precision and accuracy of the results [1]. It is still the most labor-intensive step of the analytical procedure, consuming two-thirds of the total analysis time [2,3]. This is in strong contrast to modern, fast chromatographic methods, mass spectrometric detection, and makes sample preparation a limiting step of the analysis. This is perfectly understandable if one takes into account that the application of automation sample preparation techniques reduces sample manipulation, and provides high recovery, throughput, and speed of analysis [4]
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