Solvent Effect on the Solid-Surface Fluorescence of Pyrene on Cellulose Diacetate Matrices

Svetlana M Rogacheva,Anna B Shipovskaya,Milena I Otradnova,Elena V Volkova,Tamara I Gubina

doi:10.1155/2018/3012081

Abstract

The effect of the solvent nature (acetonitrile, ethanol, dimethyl sulfoxide, and dioxane) and its concentration on the fluorescence intensity of pyrene sorbed on the cellulose diacetate (CDA) film from a water-organic solution was studied. Dimethyl sulfoxide and ethanol are shown to be the most effective solvent additives for pyrene solid-surface fluorescence (SSF). The maximum SSF signal of pyrene was found upon sorption of the substance from aqueous media containing 1.2-4.2 vol% DMSO. For the pyrene quantitation the concentration dependence of its SSF intensity at the maximum of the spectrum at λem = 394 nm and λexp = 320 nm was plotted. The dependence has a linear character in the pyrene concentration range 2·10−6 - 2·10-8 g/L, and the limit of pyrene detection is 2·10-11 g/L. The possibility of determining benzo(a)pyrene using SSF technique with the CDA matrix is proved. The proposed method is promising for use in environmental monitoring of polycyclic aromatic hydrocarbons.

Highlights

Solid-surface fluorescence (SSF) is a method of analysis which combines sorption concentration of a substance on a solid matrix followed by its fluorescent analysis in the sorbent phase [1, 2]
Earlier we reported about testing cellulose diacetate (CDA) fibers and films as matrices for the SSF of pyrene taken as a model polycyclic aromatic hydrocarbons (PAH) [21, 22]
The possibility of determining PAH in aqueous media using the method of solid-surface fluorescence on the cellulose diacetate films was proved on the example of pyrene and benzo(a)pyrene

Summary

Introduction

Solid-surface fluorescence (SSF) is a method of analysis which combines sorption concentration of a substance on a solid matrix followed by its fluorescent analysis in the sorbent phase [1, 2]. This technique provides a significant increase in the sensitivity and selectivity of fluorescence analysis. PAH take an important place among environmental pollutants because of their ability to accumulate in the environment, high stability, carcinogenic, and mutagenic activity [6, 7]. That is why PAH are found in various environmental media, materials, and food [9, 10]

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