Abstract
Abstract The retention behaviour of several iodoamino acids on μBondapak C18 columns has been investigated as the volume fraction, Ψs, of the organic solvent modifier was varied over the range 0.08<Ψs < 0.8 under low pH, low ionic strength conditions. Bimodal plots of the logarithmic capacity factor, log k′, versus Ψs were observed with selectivity reversals from a reversed phase to a polar phase elution mode occurring at Ψs ca 0.6 for acetonitrile-based eluents. The calculated slope parameters (s-values) of the iodoamino acids were similar to those found with other low molecular weight solutes but were considerably smaller than s-values observed with polypeptides or proteins of comparable capacity factor characteristics.
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