7-days of FREE Audio papers, translation & more with Prime
7-days of FREE Prime access
7-days of FREE Audio papers, translation & more with Prime
7-days of FREE Prime access
https://doi.org/10.1016/j.chroma.2017.03.037
Copy DOIJournal: Journal of Chromatography A | Publication Date: Mar 19, 2017 |
Citations: 24 |
For the first time, solvent bar microextraction using a reverse micelle RMM-SBME as extraction phase was developed for extraction of warfarin in human plasma sample. The reverse micelle of a cationic surfactant (hexadecyltrimethylammonium bromide) in 1-octanol was applied as supported and acceptor phase in SBME. In this process, the analyte was extracted from aqueous samples into a reverse micelle impregnated and also filled in the wall pores and lumen the polypropylene hollow fiber membrane by the electrostatic interactions between the analyte and the surfactant head groups. The influences of fundamental parameters affecting extraction efficiency of warfarin including sample solution pH, surfactant concentration and extraction time were studied using central composite design optimization method. The analysis of extracted analyte was performed by high performance liquid chromatography with ultra violet detector.Under the optimum condition, the sample containing 40ngmL−1 of warfarin was extracted with extraction recovery of 97.8% that pertained to enrichment factor of 260. A good linearity over a concentration range 1–600ngmL−1 with correlation coefficient value of 0.9989 was provided by the current method. Limits of detection and inner and inter-day relative standard deviation were 0.3ngmL−1 and less than 4.4%, respectively. This method was compared to conventional SBME, and illustrated higher extraction efficiency. The method needs minimal sample preparation time since the porous hollow fiber membrane prevents from being extracted potential interfering materials, acts as a filter, and any extra cleanup was not required. The method with bar of 3cm and acceptor phase volume of 8.5μL is environmentally green/friendly method. The proposed method was successfully applied for the preconcentration and determination of warfarin in plasma sample.
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.