Abstract
Experiments are described in which a standard procedure used for the ex traction of endogenous gibberellin-like substances from plants was carried out in the absence of plant material to give 'blank' extracts. Acidic, basic, and neutral fractions of blank extracts all showed variable gibberellin-like activity on d-j dwarf maize and lettuce hypocotyls, though often at a low level. Acidic fractions showed activity on d-2 dwarf maize, but none of the fractions was active on dwarf peas or on tomato seedlings. One sample of sodium bicarbonate appeared to be contaminated by two gibberellin-like substances. Some activity was observed in ethanol, chromatography paper, and ammonium acetate, but none in methanol or redistilled ethyl acetate. There were wide variations between the results of comparable experiments. In the light of these results possible precautions for experiments involving extraction of endogenous gibberellin-like substances are discussed.
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