Abstract
Solvent accessibility of different parts of a protein or a molecular complex is an important measure of its conformation and/or membrane insertion. While there are standard techniques available for bulk measurements, it is hard to do for single molecules. Here, by combining single molecule photobleaching (smPB) and fluorescence quenching techniques, we demonstrate a method that can measure the solvent exposure of membrane-attached proteins at a single molecule level. In smPB, an individual molecule or an oligomeric assembly can be characterized by counting the number of bleaching steps1,2.
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