Abstract
ABSTRACTSolvation is an indispensable part of the elution in liquid chromatography. The adsorption of solvents allows the elution of a solute from the adsorbent surface. The tendency for the adsorption of solvent molecules on the stationary phase surface results in the elution strength of a solvent. Therefore, the knowledge and the understanding of solvation process are crucial for the understanding of the retention mechanism of liquid chromatography separation. Solvation processes differ significantly between various modes of liquid chromatography (normal phase, reversed phase, and hydrophilic interaction liquid chromatography), and they may be measured using different methods. Thus, the description of solvation processes, methodologies of their measurement, and parameter-influenced solvation are reviewed and discussed.
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