Abstract
This study was aimed to extract rosmarinic acid from Orthosiphon stamineus Benth. (Lamiaceae) in high yield. The mixture of chloroform–ethyl acetate (70:30) was chosen as the solvent system because rosmarinic acid gave the lowest solvation free energy in that solvent system based on the computational solubility prediction. The crude extract of the plant was fractionated by C18 reversed phase absorbent to recover rosmarinic acid. The content of rosmarinic acid was increased from 4.0% w/w to 6.7% w/w after fractionation. The radical scavenging activity of rosmarinic acid rich fraction (IC50 = 38.3 μg/mL) was higher than the crude extract (IC50 = 58.85 μg/mL) based on the DPPH assay. Several phytochemicals were also identified based on the detection of fragment ions of target compounds. Fractions 1 to 3 could be combined to be a rosmarinic acid rich fraction. Simultaneously, the combination of fractions 4 to 6 could obtain a plant fraction rich in rosmarinic acid, sinensetin and eupatorin, whereas fractions 7 to 9 could be combined as a sinensetin rich fraction. The preparation of known phytochemical profile of O. stamineus fraction is highly required for value added product formulation and pharmacological studies, particularly for anti-diabetes and kidney related diseases which had previously been reported attributed to this herbal plant. This is the first study using solvation free energy to predict the suitable solvent system for rosmarinic acid extraction from highly complex herbal sample using the technology of solid phase extraction. The use of solvation free energy simulation is convenient and reliable before wet experiments for time and cost saving.
Highlights
Orthosiphon stamineus Benth. is a plant of genus Orthosiphon in the family Lamiaceae, which is widely used in traditional remedy to improve general health, treatment of kidney diseases, bladder inflammation, gout and diabetes in South East Asia countries [1]
Phytochemicals are known as plant secondary metabolites, which are important for plant defensive system and possess important biological activity, including antioxidant activity [4]
The mixture was incubated at room temperature for 60 min before the absorbance was read at 765 nm using an UV-Vis spectrophotometer ((UV-1800, Shimadzu Scientific Instruments, Japan)
Summary
Orthosiphon stamineus Benth. is a plant of genus Orthosiphon in the family Lamiaceae, which is widely used in traditional remedy to improve general health, treatment of kidney diseases, bladder inflammation, gout and diabetes in South East Asia countries [1]. Is a plant of genus Orthosiphon in the family Lamiaceae, which is widely used in traditional remedy to improve general health, treatment of kidney diseases, bladder inflammation, gout and diabetes in South East Asia countries [1]. The pharmacological effects of the plant might be due to the presence of several dominant phytochemicals such as terpenoids, polyphenols and sterols [2]. The phytochemicals of O. stamineus are mostly from the class of polyphenol (rosmarinic acid, caffeic acid and cichoric acid), flavonoid (sinensetin and eupatorin) and terpenoids (orthosiphol A–Z) [3]. Rosmarinic acid is one of the antioxidative plant phenolics which can be abundantly found in Lamiaceae species [5]. O. stamineus was reported to have rosmarinic acid up to 53 mg/g extract [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
