Solution State Structure of pA1, the Mimotopic Peptide of Apolipoprotein A-I, by NMR Spectroscopy

Abstract

E-mail: hswon@hanyang.ac.krReceived July 31, 2011, Accepted September 20, 2011A number of mimetic peptides of apolipoprotein A-I, a major component for high density lipoproteins (HDL),were screened from the phase-displayed random peptide library by utilizing monoclonal antibodies (A12). Amimetic peptide for A12 epitope against apolipoprotein A-I was selected as FVLVRDTFPSSVCCP(pA2)exhibiting 45% homology with Apo A-I in the BLAST search. Solution structure determination of thismimotope was made by using 2D-NMR data and NMR-based distance geometry (DG)/molecular dynamiccalculations. The resulting DG structures had low penalty value of 0.4-0.6 A