Abstract
Quantitative evaluation of elution volumes ( V e) of polyelectrolytes in salt-containing eluents was performed, taking into account electric double-layer effects and the effective radius of polyions, and assuming that polyelectrolytes behave as rigid hydrodynamic spheres and that the geometry of gel pores is cylindrical. A polynomial V e dependence on ionic strength ( I) was obtained, namely a second order one in I − 1 2 . The semi-empirical polynomial coefficients depend on column characteristics, namely V 0 (interstitial volume), V p (total pore volume) and a (mean pore radius), as well as on effective coil radius ( R η) and on k (a parameter related to the electric effects). Fair correlations between predicted and measured elution volumes for uncharged polymers hold, at least for the polyelectrolyte—gel systems tested here: polyelectrolytes poly( l-glutamic acid), sodium poly(styrene sulphonate) and poly(acrylic acid); gels Spherogel TSK PW4000 and Ultrahydrogel 250.
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