Solution and solid-state 33 S NMR studies of 33 S-labeled taurine.

Abstract

Sulfur-33(33 S) stable-isotope labeled taurine, 2-aminoethanesulfonic acid, has been synthesized, and a series of solution and solid-state 33 S nuclear magnetic resonance (NMR) experiments at 14.1 and 18.8T, respectively, have been carried out at room temperature. The single peak of a solution 33 S NMR spectrum in 0.1-mM [33 S]-taurine in D2 O can be observed with the signal-to-noise (S/N) ratio of 9 in 40,000 scans, which paves the way toward in vivo analysis of pharmacokinetics and metabolism of 33 S-labeled taurine. Undistorted magic-angle-spinning (MAS) and static 33 S NMR spectra of polycrystalline [33 S]-taurine are observed with sufficient S/N ratios for analysis, and the magnitudes of 33 S EFG and CS tensors can be obtained.