Solute production and net wall synthesis in the growing and non-growing cells of gravistimulated sunflower hypocotyls

Abstract

Solute generation and cell wall synthesis were examined in sunflower hypocotyl peripheral layers, the growth rate of which had been altered by gravistimulation. Measurements of both the concentrations of the major solutes and the osmotic potential showed that although upper cells stopped growing, the solute levels in these cells continued to increase at rates comparable to those in lower cells. This indicated that altered growth rates, generated during gravicurvature, are not based on solute generation but must result from cell wall changes. Gravimetric and precursor incorporation studies showed that net wall synthesis continued in upper cells despite their lack of growth. An ultrastructural study of the epidermal cells on the uppermost (non-elongating) and lowermost (elongating) surfaces of horizontal cucumber hypocotyls showed that the relative amounts of the various membrane fractions were similar in upper and lower cells despite their very different growth rates.