Solute levels and osmoregulatory enzyme activities in reed plants adapted to drought and saline habitats

Abstract

The pattern of solute accumulation and the activities of key enzymes involved in metabolism of proline and betaine were investigated in three ecotypes of reed from different habitats: swamp reed (SR), dune reed (DR), and heavy salt meadow reed (HSR). The two terrestrial reed ecotypes, DR and HSR, exhibited a higher capacity for osmotic adjustment; they accumulated higher contents of K+ and Ca2+ in the leaves in comparison with SR. DR also had the highest soluble sugar content in its leaves. HSR has higher levels of Na+ in its root environment and this was reflected by considerable accumulation of Na+ in the HSR rhizome. However, the different zones of its leaf lamina (upper, middle and lower) did not exhibit increased levels of Na+, suggesting that HSR has the ability to accumulate Na+ in the rhizome to protect the shoots from excessive Na+ toxicity. DR and HSR had higher levels of proline and betaine in the leaves than did SR. This difference was consistent with the activities of the various biosynthetic enzymes: betaine aldehyde dehydrogenase (BADH), pyrroline-5-carboxylate reductase (P5CR) and ornithine-δ-aminotransferase (OAT) were enhanced in DR and HSR as compared to SR, whereas proline oxidase (PO) activities were inhibited. These findings suggest that changes in the activities of enzymes involved in osmotregulation might play important roles in the adaptation of reed, a hydrophilic plant, to more extreme dune and saline habitats. The relative contributions of the various proline synthetic pathways are also discussed.