Abstract
The level of soluble superoxide dismutase (E.G.1.15.1.1) was determined in Phaseolus vulgaris leaves susceptible (cv. Pinto 111) and hypersensitive resistant (cvs K.W. 765 and K.W. 814) to Uromyces phaseoli. In all cultivars tested increased levels of extracted Superoxide dismutase (SOD) appeared to be associated with the appearance of flecks and the differentiation of the uredosori, in the susceptible cultivar, and of microscopic or macroscopic necrotic lesions in the hypersensitive cultivars. Different SOD enzymes (Mn or Cu, Zn-SOD) were selectively stimulated by compatible and incompatible host-pathogen interactions. There was an increase in activity of the Mn-enzyme in the susceptible leaves during stroma differentiation in the mesophyll. By contrast there was an increase in activity of the Cu,Zn-enzyme in the hypersensitive response of resistant leaves. The above mentioned increases were due to a host response to infection since the contribution of fungal SOD was negligible. SOD and peroxidase increased simultaneously. The increase of peroxidasr greatly exceeded that of SOD only in the hypersensitive cultivars. The infection did not change the electrophoretic behaviour of SOD enzymes.
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