Abstract
Abstract The paramagnetic contributions induced by TEMPOL soluble spin-label on gentiobiose carbon spin-lattice relaxation rates are analyzed. Selective effects in the β(1–6) glycosidic bond region were observed. The possibility of using soluble spin-label to determine the stereochemistry of the substrate-enzyme interaction was then explored. The results obtained with different diamagnetic and paramagnetic systems enabled us to distinguish the region of gentiobiose most involved in interaction with the enzyme, and the region of the disaccharide molecule located on the surface of the enzyme and most exposed to the nitroxide. The results obtained could be used to model the enzyme surface of the gentiobiose binding site. Soluble spin-labels have been widely used in the investigation of the conformational and dynamical properties of biomolecules in solution (1–4). The main effects of nit oxide spin-labels, like TEMPO or TEMPOL, is the increase in NMR spin-lattice relaxation rates of solvent exposed nuclei. In...
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