Soluble I131fibrin form high molecular weight complexes in prothrombin‐depleted, recalcified plasma at +37 °C

Abstract

Gel filtration of plasma samples containing I125fibrinogen and either I131des‐AA soluble fibrin or I131des‐AABB soluble fibrin, were performed on Sepharose 6B C1 columns at + 37 °C, equilibrated and developed with prothrombin‐depleted plasma, either recalcified or containing EDTA. With EDTA, des‐AA fibrin eluted in a homogenous, monomeric form, while des‐AABB fibrin had an elution profile indicating high molecular weight derivatives. In the presence of calcium ions, both des‐AA and des‐AABB soluble fibrin eluted mainly as high molecular weight derivatives. It could not, however, be decided whether the high molecular weight derivatives consisted of fibrin molecules alone, or of co‐polymers of fibrin‐fibrinogen. It is concluded that soluble des‐AA fibrin and soluble des‐AABB fibrin in plasma at + 37 °C and in the presence of calcium ions, participate in the formation of high molecular weight derivatives of fibrin.