Soluble guanylate cyclase crystal clear: 1stcrystal structure of the wild-type human heterodimeric sGC catalytic domains and implications for activity

Abstract

Background Soluble guanylate cyclase (sGC) is the key enzyme in the NO-sGC-cGMP signaling cascade crucial in regulating the cardiovascular system. Low output of this system causes hypertension and acute heart failure, which are the leading causes of death globally. Mammalian sGC is a heterodimer of two homologous subunits (a and b), which contain four domains: an Nterminal regulatory domain (HNOX: Heme Nitric oxide OXygen), an HNOX associated (HNOXA) domain and a coiled-coil (CC) domain important for dimerization, and a C-terminal catalytic domain (GC) (Figure 1). The enzyme is basally active, but NO binding to the heme group in the b subunit regulatory domain enhances sGC catalytic output several hundred fold. The molecular mechanism by which the regulatory domain relays the activation signal to the catalytic domain remains elusive. Several studies have highlighted the crucial role of the HNOXA and CC domains for sGC dimerization necessary for catalytic activity [1-9]. Others have shown that the