Abstract
We have developed a simple, rapid, and sensitive enzyme-linked immunoadsorbent assay (ELISA) to measure soluble cell-free human Fcγ receptor (FcγR) in serum. This assay is based on the use of two monoclonal antibodies directed against different epitopes expressed on the same low avidity human FcγR (CD16), which is present on polymorphonuclear leukocytes, macrophages and NK cells. This sandwich ELISA, which can measure 2 nM concentration of FcγR, has enabled us to demonstrate the presence and to measure the level of soluble cell-free human FcγR (CfH-FcγR) in normal human serum.
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