Abstract
CD44, a widely distributed cell surface glycoprotein and a receptor for hyaluronan (HA) has been implicated in facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells. CD44 shedding has been observed in different tumor cell types but not using cell lines derived from colorectal cancer. In order to investigate the role of soluble CD44 (CD44 sol) in colon cancer cell growth, SW620, a human colon cancer cell line deficient in CD44 standard isoform expression was stably transfected with human CD44 cDNA containing exons 1–5, 15 and 16 of the human CD44. Western blot analyses demonstrated the presence of 78 kDa soluble CD44 protein in the culture supernatant of stably transfected cell lines as compared to control cells transfected with the empty vector control. These CD44 sol transfected cells showed higher cell proliferation and clonal growth in vitro, confirmed by MTT and clonogenic assays respectively, when compared to control cells. Cell adhesion to HA was significantly lower with CD44 sol clones compared to control cells. Western blot analyses for cleaved PARP were negative in lysates from CD44 sol cells suggesting resistance to apoptosis. These findings indicate that the secretion of soluble CD44 contributes to colon cancer growth, possibly as a decoy receptor.
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