Abstract
It was confirmed that the nuclear testosterone 5α-reductase was attached to membrane fraction as observed by Moore and Wilson (1972) in rat ventral prostate. Testosterone 5α-reductase of the nuclear membrane fraction was partially solubilized by phospholipase A, and overall 20 fold purification was attained. Optimum pH, pyridine nucleotide requirement and time course of the reaction of the solubilized enzyme were examined.
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