Abstract
1. 1. Different non-hydrolytic procedures for the solubilization of NADH-cytochrome b 5 reductase and cytochrome b 5 from Ceratitis capitata larvae microsomes have been compared. 2. 2. Solubilization of both proteins by detergents was a more adequate method than detergent extraction after delipidation with acetone of native microsomes or phosphate buffer extraction. Treatment with acetone produced an irreversible denaturation of these proteins. 3. 3. CHAPS was the most effective of all detergents assayed in the solubilization of cytochrome b 5 whereas Triton X-100 and Triton N-101 were more suitable for the solubilization of NADH-cytochrome b 5 reductase. The relation between solubilizing power and HLB number of non-ionic detergents is discussed. 4. 4. CHAPS appears to be efficient in breaking protein-protein interactions and thus at 6 mM induced disaggregation of purified NADH-cytochrome b 5 reductase from the insect to a dimeric form.
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