Solubilization of anion ATPase from necturus oxyntic cells

Abstract

An SCN −-inhibited, HCO 3 −-stimulated ATPase (ATP phosphohydrolase, EC 3.1.6.3) has been shown to be localized in the oxyntic cells of Necturus gastric mucosa. Carbonic anhydrase is also associated with this cell type. High activity of the ATPase was found in a pure smooth microsomal fraction of a homogenate of these oxyntic cells. A method is described for solubilization of the enzyme resulting in a marked increase in the sensitivity to HCO 3 − and SCN −. High activity of the enzyme is found in tissues of Necturus which transport H + and/or C1 − and which have a high carbonic anhydrase content.