Abstract
Effects of temperature and d-cis-diltiazem (DTZ) on [ 3H]nitrendipine (NTD) and [ 3H]nimodipine (NIM) binding to skeletal muscle t-tubular membranes were studied. A decrease in temperature from 37°C to 10°C decreased K D and increased B max slightly. DTZ increased binding by increasing B max under all conditions and also decreased K D for NTD at 37°C. The binding protein labeled with [ 3H]isothiocyanate dihydropyridine revealed a molecular weight of 36,000. The binding site for NTD was solubilized by deoxycholate and dihydropyridine binding was still stimulated by DTZ in the solubilized form.
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