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Abstract
Solubility of human skin collagen was studied by extracting skin specimens with o.14 MNaCl solution. The extraction conditions were studied by varying the time and number of extractions. A method combining pooled supernatants from three successive extractions of 24 h was chosen for standard procedure of extracting the neutral salt-soluble fraction from human skin collagen. Using this method, the solubility of skin collagen in biopsies taken from 70 control subjects of various ages and from 27 patients with generalised scleroderma was determined. No changes in the degree of solubility during ageing could be observed, whereas the solubility in the skin of patients with scleroderma was increased.
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