Solid‐State NMR Spectroscopy of a Paramagnetic Protein: Assignment and Study of Human Dimeric Oxidized CuII–ZnII Superoxide Dismutase (SOD)

Abstract

A solid story: The paramagnetic form of the protein superoxide dismutase (SOD; see portion of structure in upper right corner of picture with detectability sphere in blue) is shown to be accessible to high-resolution solid-state magic angle spinning NMR studies when in microcrystalline form. A nearly complete assignment of the signals of this 32-kDa dimer has been achieved (13C–15N NMR correlation spectrum shown in background). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z603093_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.