Solid-State Fermentation in Brewer’s Spent Grains by Fusarium fujikuroi for Gibberellic Acid Production

doi:10.33263/briac115.1304213052

Abstract

The search for new production methodologies of gibberellic acid (GA3), such as solid-state fermentation (SSF), and the use of agro-industrial waste are important to lower production costs. Therefore, the aim of this study was GA3 production by Fusarium fujikuroi on SSF mode using brewer’s spent grains (BSG). BSG presents in its composition components that are known to be excellent inducers of metabolite production, showing, this way, its potential to be used as the substrate in biotechnological processes. Optimization of GA3 production was carried out using a 22 central composite design, considering the effects of moisture content, temperature, and fermentation time. The highest mycelial growth and GA3 production (0.82 g.Kg-1) was obtained in the condition of 80% moisture content, 28 °C in 96 hours of fermentation. These results suggest that the SSF using BSG as the medium for the growth of F. fujikuroi is a viable way to GA3 produce.

Highlights

Gibberellins are phytohormones resulting from secondary metabolites
Microbial growth in the brewer’s spent grain (BSG) was determined according to the adapted Standard Methods G21-15 [43]: 0 - absence of growth; 1 - little growth, small fragments of mycelium thrown into the culture medium; 2 - moderate growth, the appearance of fine pellet on the surface of the culture medium; 3 - great growth, the appearance of mycelium in more than half of the medium
The results agree with the literature where, for Santos et al [22], the moisture was between 8.7 and 10.8%, protein content 24.2%, fat content 3.9%, and ash content 3.4%

Summary

Introduction

Gibberellins are phytohormones resulting from secondary metabolites They are important biotechnological products, with great economic value, used in agriculture as natural plant growth hormones [1]. They play a role in breaking flowering dormancy, increasing flowering initiation, increasing stunted plants, spurring germination processes [2-5]. Studies have focused on increasing yield, productivity, and, mainly, reducing production costs so that its use is not restricted

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Conclusion