Abstract

There has been renewed interest in using algae for wastewater polishing and treatment in recent years. Because solids retention time (SRT) is a key design and operating parameter in bioreactor operation, this research determined the effect of SRT on phototrophic growth and microbial population dynamics in continuous-flow chemostat systems. There was a unique feature of phototrophic growth that differed from chemotrophic growth in chemostat. It was found that the phototrophic biomass concentration increased proportionally as SRT increased from 3 to 9 days. Regardless of the change in SRT, a step function model was successfully applied with the predicted phototrophic production rate of 4.5 ± 0.9 g/m(2)·d at the light intensity of 68.5 μmol/m(2)·s. Even though the continuous-flow systems were initially seeded with a 1:1 mixture of green algae and cyanobacteria, Chlorella vulgaris always dominated (98%) in the continuous-flow chemostat systems under steady-state conditions.

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