Abstract
Abstract In this paper, we discuss two subjects concerned with solid phase extraction (SPE) and HPLC analysis of toxic compounds eluted to serum from materials used for medical devices. SPE and liquid-liquid extraction for serum toxic compounds were compared. Firstly, an analysis of a toxic and carcinogenic compound, 4,4′-methylenedianiline (MDA), from polyurethane sterilized by gamma-ray irradiation was studied. Serum MDA was analyzed to assess the potential MDA risk to patients exposed to PU medical devices. MDA was determined by reverse-phase HPLC using an eluent of a mixed solution of ammonium acetate and acetonitrile. An ODS column was used. Detection was carried out by an electrochemical detector (ECD). As liquid-liquid extraction was complex due to deproteinization followed by centrifuge separation and concentration, we substituted SPE. Serum MDA was satisfactorily recovered using Bond Elut C-18, Phenyl and Cyclohexyl columns. The recovery of serum MDA from Bond ElutR C-1, C-2, C-8 and Silica was u...
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