Abstract
A simple yet highly efficient pretreatment method called solid-phase dispersive extraction (SPDE) was developed and used in combination with liquid chromatography/time-of-flight mass spectrometry (LC/TOF-MS) for the analysis of benzodiazepines (BZPs) in serum and urine samples. By using a custom-made centrifugal filter, SPDE could be performed in a closed system, thereby minimizing exposure to infectious microbes or hazardous chemicals. The limit of detection and the limit of quantification of nine BZPs were 1–10 and 5–50ng/mL, respectively. The average recoveries of BZPs from pooled serum samples spiked at 50 and 500ng/mL were 89.6–105.0% (RSD: 2.1–6.8%) and 93.6–110.4% (RSD: 2.1–4.2%), respectively, and those from urine samples were 88.7–105.5% (RSD: 2.9–6.4%) and 91.5–101.1% (RSD: 3.6–5.5%), respectively. SPDE-LC/TOF-MS has potential application in forensic science and emergency medicine.
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